High throughput screening of the potential biosurfactants production by extremophiles isolated from vinasse and black liquor

The aims of this work were to isolate microorganisms from vinasse (V) and black liquor (BL), by-products alcohol paper industries assess their potential as substrates produce biosurfactants. Thirty-nine microorganisms, 19 V (acidophilic, a1 a19) 20 BL (alkaliphilic, b1 b20) isolated. A high- throughput method was design set up for surface tension ( γ ) measurement. In the first screening, 15/39 strains pre-selected capacity reduction media formulated with BL. Student's t -test applied data obtained in second search significant differences among values reached each production respective sterile medium. Three acidophilic (a1, a5, a6) 3 alkaliphilic (b1, b2, b17) significantly decreased compared control p < 0.05) after 5 days 5% substrate concentration. BC index defined biosurfactant capacity. showed following percentage reduction: Lactobacillus rhamnosus a5 (34%), Bacillus safensis (13%), Alkalihalobacillus halodurans b2 (10%) Pichia cecembensis a6 (10%), paracasei (6%). two that presented best greatest when -value) = 16.98, 0.007), 17.66, 0.003). These industrial are a source new super-producing will be economic production. • Microorganisms isolated can potentially Vinasse high screening proposed reduction. Strains bacteria yeast reduce sp. produced lowest growth